Li Y, Guan Z, Yan J
[Screening cellular proteins interacted with M2 protein of influenza A virus by coimmunoprecipitation] [English Abstract, Journal Article, Research Support, Non-U.S. Gov't]
Wei Sheng Wu Xue Bao 2009 Aug 4; 49(8):1081-5.
OBJECTIVE: To screen cellular protein interacted with influenza A M2 protein (A/M2).
METHODS: We cloned A/M2 gene fragment into pCAGGS-CFlag vector, and the resulting plasmid was transfected into human embryonic kidney (HEK) 293T cells.The recombinant Flag fusion protein, A/M2-Flag was absorbed specifically by Anti-Flag Monoclonal Antibody M2-Conjugated Agarose beads, we loaded the beads on 12% SDS-PAGE after we washed it with lysis buffer. Silver staining of the gel revealed that several proteins were co-purified with A/M2. To identify the proteins, we excised the protein bands and analysed them by mass spectroscopic sequencing.
RESULTS: We got two kinds of proteins, ataxin 10 and eukaryotic initiation factors (eIFs).
CONCLUSION: Interaction between Ataxin 10 and A/M2 would explain why influenza virus infection or influenza vaccine innoculation causes acute cerebellar ataxia. A/M2 interacting with eIFs would imply that A/M2 is involved in the regulation of influenza virus protein synthesis.
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